AB081. Research on epididymis inflammatory mass in 1,021 patients with varicocele
نویسندگان
چکیده
© Translational Andrology and Urology. All rights reserved. Transl Androl Urol, 2017;6(S3) tau.amegroups.com 17α-hydroxylase were examined by western blotting and RT-PCR, respectively. Annexin A5 significantly stimulated testosterone secretion from rat Leydig cells in doseand time-dependent manners and increased mRNA and protein expression of StAR, P450scc, 3β-HSD and 17β-HSD but not 17α-hydroxylase. Annexin A5 knockdown by siRNA significantly decreased the level of testosterone and protein expression of P450scc, 3β-HSD and 17β-HSD. The significant activation of ERK1/2 signaling was observed at 5, 10, and 30 min after annexin A5 treatment. After the pretreatment of Leydig cells with ERK inhibitor PD98059 (50 μmol/L) for 20 min, the effects of annexin A5 on promoting testosterone secretion and increasing the expression of P450scc, 3β-HSD and 17βHSD were completely abrogated (P<0.05). Thus, ERK1/2 signaling is involved in the roles of annexin A5 in mediating testosterone production and the expression of P450scc, 3β-HSD and 17β-HSD in Leydig cells.
منابع مشابه
Role of Ultrasound and Colour Doppler in Scrotal Pain
100 patients coming to JSS with scrotal pain between November 2011 and August 2014 were studied. Each patient was examined in multiple planes using 7.5MHz-10MHz variable frequencies with colour and pulse Doppler 5MHz frequency probe was used in a few cases who had large scrotal mass. Most of the patients in our study belong to age group between 20-40 years (68%).In our study, Inflammatory condi...
متن کاملP-11: Evaluation of Heat Shock Protein A2 in Male Rats before and after Varicocele Induction
Background: Varicocele is a major cause of male infertility, as it may impair spermatogenesis through several distinct pathophysiological mechanisms including hypoxia, renal-adrenal reflux, hormonal dysfunction, autoimmunity, oxidative stress and hyperthermia. HSP70s are members of the heat shock protein (HSP) family, which are considered intracellular chaperones. Their expressions can increase...
متن کاملP-17: Body Mass Index and Sperm Counts Associated with Varicocele in Infertile Men
Background: Varicocele is a progressive disease and etiology of varicocele is not well known. Some studies have been reported that prevalence of varicocele decreases with increasing BMI. The aim of this study was to determine interrelation of varicocoele with height, body mass index (BMI) and sperm counts in infertile men. Materials and Methods: We retrospectively evaluated the data of all pati...
متن کاملO-17: Study of Sperm Protein Profile in Oigo- and Normozoospermic Patients with Varicocele Using Two Dimensional Eectrophoresis
Background: The aim of this study was to compare the sperm protein profile between oligo- and normozoospermic patients with varicocele. Materials and Methods: Sperm samples of 20 normozoospermic and 20 oligozoospermic patients with varicocele were characterized using two dimensional electrophoresis (group 1 and group 2, respectively). Differences in protein expression were established using gel...
متن کاملTreatment of Bilateral Varicocele and Other Scrotal Comorbidities Using a Single Scrotal Access: Our Experience on 34 Patients
INTRODUCTION Varicocele is the main cause of infertility in male and the most correctable cause of it too. In this study, we present our experience on 34 patients affected by bilateral varicocele and other scrotal comorbidities treated underwent surgery with a scrotal access. MATERIALS AND METHODS 34 patients were enrolled with clinical palpable and infraclinical (ultrasonic doppler scanning)...
متن کامل